The healthcare service area while providing services, curative, primitive or preventive inevitably create waste which itself may be harmful to health. It carries a higher latent for infection and injury than any other type of waste. Inadequate and inappropriate knowledge of handling of healthcare waste may have serious health penalties and a significant impact on the environment (Suwarna Madhukumar April 2012)... Insufficient and inappropriate knowledge of waste and handling of waste may have serious health complications. To determine awareness of infectious waste management among staff nurses and describes their level of knowledge, attitude and practice towards infectious waste management. A cross-sectional analytical study design was used to observe the knowledge, attitude and practice of staff nurses. A cross-sectional is that study that collects information from a population at specific time of period (Lisa B 2014). Data was collected from 50 nursing staff through convenient sampling techniques. Those who meet the inclusion criteria will be included in the study and those who don't meet the criteria were not being the part of the study. Data was analyzed through SPSS version 16. Results suggests that there was unsatisfactory knowledge among nursing staff of mayo hospital Lahore.50% of the study participants had knowledge about infectious waste management , other 50% had poor knowledge regarding waste management techniques and procedures. Red container is used for cytotoxic items. Out of 50, only twenty six (52%) study subjects answered full destruction of all cytotoxic substances require incineration at high temp up to 1200 degree centigrade.30%subjects did not comment. Participants had poor practice related to sharp items wasted only 19% participants knows yellow puncture proof container is used for sharp items. Forty two (84%) Nurses were aware about containers should be filled three quarters full and then disposed. Only 8%nurses knows Hepatitis B is greatest risk from infectious hazard.54%nurses assume that Hepatitis C is greatest risk. The attitude of the study subjects toward awareness of waste management, separation of infectious and noninfectious waste, proper disposal and implementation of rules was positive. According to my results nursing staff had poor knowledge so that their practice was also poor and unsatisfactory .they all need regular training and monitoring.