Correct body positioning is a major nursing intervention to prevent respiratory problem and reduce complication. The important causes of neonatal deaths is birth asphyxia and along with serious infections results in more than 50% of all neonatal deaths.The study was conducted to assess the effect of positioning on physiological parameters of neonates admitted with respiratory problem and find out the difference among positions. The conceptual frame work of the study was based on Stuffle Beams evaluation model provides a framework for evaluation of an innovative intervention .The research design adopted was quasi experimental with one group post test design. The experiment was done by taking 60 neonates admitted for respiratory problem. The level of significance was found using “Z’’ test & ANOVA.The mean heart rate in prone position is 132.4  15.26 less than mean heart rate in lateral position is 137.8  9.82 and in supine position is 141.6  9.75. The mean oxygen saturation in prone position is 95.9  2.87 is higher than the mean oxygen saturation in lateral position is 88.9  2.49 and in supine position is 91.2  2.54. The mean respiration rate in prone position is 43.8  3.18 is less than in lateral position 51.4  4.33 and supine position is 52.2  1.9. The mean arterial pressure in supine position is 49.1  8.27 is same as in prone position is 49.1  8.12 and in lateral position is 48.56  7.57.The mean of oxygen saturation, respiration rate, heart rate in prone position is clinically effective. Prone position improves oxygen saturation and decrease respiratory distress in compared to supine and lateral position in neonates having respiratory problems.