Nurses are present at both the beginning and the end of life, and play a key role in caring for dying patients. It clearly indicates that role of nurses can never be ignored when we are talking of end of life care or palliative care. Palliative care is defined by literature as care that improves the quality of living in individuals who are facing life threatening problems and illness to prevent and treat them through early identification of pain and other physical and psychosocial problems. The main purpose of this study was, to assess palliative care knowledge and attitude among nurses of Mayo Hospital Lahore. A cross sectional descriptive survey was performed to assess the knowledge and attitude level. A simple random sample of n=150 was used. A structured questionnaire with demographic data, knowledge and attitude of palliative care was applied. The data was analyzed on SPSS version 21. 36% of the study participants had poor knowledge, 49.33% of the study participants had average knowledge and very less number of participants that is only 14.7% of the study participants had good knowledge of palliative care. Findings reveals that 33.33% of the study participants had negative attitude towards care of end of life among terminally ill patients, while 66.7% had positive attitude towards end of life care. Knowledge was poor among majority of the participants but attitude was found positive among them