The main objective of the present study was to relate the urine urobillinogen with tooth decay. It is a colorless substance that is formed by the reduction of the bilirubin and bilirubin is a yellowish substance that is formed by the breakdown of the red blood cells. So urobillinogen is a substance that is formed by the bacterial attack upon the bilirubin in the intestine (duodenum). A total of 100 subjects get involved in this study from Bahauddin Zakariya University Multan, Pakistan. After taking the results we designed a project based upon these results. In this some had normal percentage and some had 1 or 0.1 percentage. In this, females that had negative percentage were 10% and had positive 55% in the case of when they had tooth decay. While values for females were in case of when they had no tooth decay for negative percentage were 11% and for positive percentage were 25%. In case of males that had tooth decay were 10% in negative case, while had 58% in positive case. When males had no tooth decay value for negative were 11% and for positive percentage were 25%. It was concluded that there was no relation among tooth decay and urobillinogen.