The chest tube is used to restore the intra pleural pressure and to prevent the collapse of lungs. Chest tube management includes the actions to keep the tube functioning properly, which is the prime role of nurses while caring of patients with chest tube drainage Elfeky, 2013. The main objective of this study was to determine the level of knowledge regarding chest drains care among staff nurses of Mayo hospital Lahore. A cross-sectional descriptive study design was used to assess the level of nurses’ knowledge regarding chest drains care. A Convenient sample technique was used in this research study. A sample of n=150 was recruited. The main instrument for Information was questionnaire adopted from the research (Danish Ruhel 2013). The questionnaires were divided into two sections. Section 1 comprised of demographic information of respondents; Section two consist of knowledge based questions. Data was analyzed through SPSS version 21. The results are shown below. The finding of this study shows that among the participants 24% had poor knowledge of chest drainage care and they answered 13 or less correct answers off the 23 questions being asked. 24% of the participants had moderate knowledge and scored 14-19 correct responses, while 40% of the study participants had good knowledge of drainage care and answered above 19 correct responses. Overall there was a satisfactory level of knowledge regarding the chest tube drainage care among nurses of the selected hospital in Lahore.