Saudi Journal of Biomedical Research (SJBR)
Volume-4 | Issue-03 | 110-114
Original Research Article
Evaluation of the Immunochromatographic Strip Method and Microscopic Method in the Identification of Malaria Parasites
Chukwurah Ejike Felix, Obeagu Emmanuel Ifeanyi, Ututu Sunday Unahci
Published : March 31, 2019
Abstract
This project work evaluated the strip (rapid diagnostic test) method and microscopic method in the identification of
malaria parasites. To evaluate the suitability of both methods and also to find the advantage and disadvantage of both
methods. Two ml (2ml) blood sample was collected with a total number of 100 samples from children between the age of
5-14years presented with fever attending Federal Teaching Hospital Abakaliki, haematology/blood group serology unit
into EDTA container in the basis of first come first serve were used. Out of the 100 patients under present study, 71%
turned out to be positive as overall. Out of these, 59% were positive for strip method whereas 71% were positive for
microscopic method (thick film). Both methods showed that the most pathogenic and predominant species in Abakaliki is
plasmodium falciparum. Thick film was made in a clean grease free slide immediately after collection and allowed to air
dry and stained with Giemsa stain diluted with buffered water (pH 7.2) for 20 minutes and the stained slide was viewed
microscopically by focusing with XI0 and examine with XI00 objective (oil in immersion). Strip method (rapid
diagnostic test) used is manufactured from standard diagnostics, INC. (SD). The test device was removed from the foil
pouch, placed on a flat, dry surface. With the aid of a 5ul capillary pipette provided, draw whole blood to a black line and
then transfer the drawn whole blood into the round sample well. Four drops of assay diluents was added vertically into
the square assay diluent well. Result was read after 15 minutes, one line (control line) indicate negative result while two
lines test and control line indicate positive result. Both methods are good in the identification of malaria parasites, but
microscopy is the “gold standard”. Microscopic method requires high skill manpower, a lot of training/expertise and are
not encouraged in rural area with low/no power supply. Strip method is rapid, does not requires expertise/training and it
does not consume time, but it is just a screening test, if used should be confirmed with the microscopic method.