Ventilator associated pneumonia is the most common nosocomial infection arising as complication at Intensive care units. It affects nearly 8-20% of the clients admitted at intensive care units and is 27% among the mechanically ventilated patients (Al Shameri). The main purpose of this study was, to assess the knowledge and practice of Ventilator associated Pneumonia prevention among nurses at PIC Hospital Lahore. A cross sectional descriptive survey was performed to assess the knowledge and practices among Nurses. A convenient sample of n=122was used to collect the data. A structured adopted questionnaire was used to collect data. The questionnaire consisted demographic variables, Knowledge of VAP and Practices of VAP among the nurses of the ICU at PIC Lahore. The data was analyzed on SPSS version 21. Overall knowledge was satisfactory where 50% of the study participants had good knowledge and gave more than 80% correct responses about different aspects of the Ventilator Associated pneumonia prevention. 30.33% were satisfactory knowledge about the VAP prevention. Overall Practice result very good practices among 83% of the participants who were practicing 20 or more items out of 24 statements. 12.33% had satisfactory practices. Overall knowledge was satisfactory and practices were good among the nurses at PIC regarding ventilator associated Pneumonia.