Catheter Associated Urinary Tract Infection (CAUTI) is the most common healthcare associated infection arising as the complication at intensive care units accounting for 80% of all hospital acquired infections .Urinary tract infections (UTI) are the most commonly reported Health Associated Infections (HAIs) in the United States, accounting for 32% of all infections Weber, Sickbert-Bennett et al., 2011. Although many preventive measures and guidelines to prevent CAUTI exist in different health care setting. The main purpose of this study was, to assess the knowledge and attitude of nurses towards the prevention of catheter associated urinary tract infection in the ICU’s of the public hospitals Lahore. A cross sectional descriptive survey was performed to assess the knowledge and attitude among Nurses. A convenient sample of n=160 was used to collect the data. A structured adopted questionnaire from knowledge, attitude and practice of nurses towards the prevention of catheter associated urinary tract infection in selected referral hospitals in rwanda was used to collect data. The questionnaire consisted demographic variables, Knowledge and attitude of nurses about CAUTI among the nurses of the ICU at public hospitals Lahore. The findings reveals that majority of participants 48.75% were having moderate knowledge towards CAUTI prevention, having 48.13% poor knowledge and 3.13% having only good knowledge. However, attitude of nurses toward CAUTI prevention was 60% negative and 40% positive. The data was analyzed on SPSS version 21. Overall knowledge is satisfactory however negative attitude needs much attention.