The present study investigates the comparison of hand hygienic practices in nurses of neonate and pediatric unit allied hospital and DHQ hospital Faisalabad. Hand hygiene is a primary measure to reduce infection in neonates. Hand hygiene is a simple procedure. That can help in reducing morbidity and mortality rate. The purpose of this study is to comparison of hand hygienic practices in nurses of neonate and pediatric unit Allied hospital and DHQ hospital Faisalabad. To assess nurses compliance of hand hygiene related to new born care. To assess association between hand hygiene training and hand washing practice. The study’s methodology involved descriptive quantitative study design. Sample size was 180. Questionnaires were distributed to the nurses of two hospitals. 150 questionnaires returned back. Questionnaire was containing in 3 sections. The data analysis was done using SPSS software version 21. Results describes about I receive formal training for hand hygiene. Results indicate that 100% respondents were Agree with the statement. The mean is 1.0000 with .00000 standard deviation. Results describes about There is a relationship between good hand hygiene practice and hospital acquired infections. Results indicate that 88.0% respondents were strongly agree, 12.0% agree with the statement. The mean is 1.1200 with .32605 standard deviation. Results describes my clinical nursing instructor consistently performs HH when necessary. Results indicate that 65.3% respondents were strongly agree, 28.0% Agree and the UN decided 6.7% with the statement. Hand washing is a primary measure to reduce infection and prevent spread of health care associated infection. Descriptive quantitative design used for this study. Data was collected through adopted questionnaire Descriptive statistics was used for analyzing date.