Background: It was an experimental comparative study conducted in Sudan, faculty of healthy sciences at Alsheikh Abdallah Elbadri University, among 144 Sudanese and non-Sudanese volunteers, age between 16 to 49 years old. ECG was done before and after exposure to mobile cell phone and both results was compared. The mobile cell phone was placed on chest pocket level during silent modes. Objectives: The study was aimed to detect the effect of silent mobile phone electromagnetic radiation on ECG parameters in both genders. Results: ECG parameters mean +STD of P, PR, ST and QT have significant change within normal range correlated with heart rate and rhythm, while the mean +STD of QRS duration and T wave has no significant changes. Conclusion: The mobile phone wave during silent mode has direct effect on ECG parameters which leads to sinus arrhythmia.