Objectives: To assess oral health status and prevalence of obesity, hypertension and diabetes mellitus and sleep related disorders in bus conductors and drivers. Method: Cross-sectional survey was conducted at interstate bus terminal of Lucknow district. Total 156 bus conductors and drivers were enrolled in this study. Oral health status was assessed by WHO oral health questionnaire and prevalence of obesity, hypertension, diabetes and sleep related disorders were recorded using standardized questionnaire and biochemical investigations. Data was analysed using SPSS version 25.0 at significance level of 0.05. Result: The mean systolic and diastolic blood pressure was found 127.1 and 85.3 respectively for all the subjects. Mean value was observed 36.9 cm for neck circumference, a mean score of 3.1 was found for mallampati grading and a mean score of 5.9 was observed for Epworth sleepiness score for all the studied subjects. The mean value for Serum Cholesterol, triglyceride and HDL-C were found 174.7, 153.8 and 54.5 mg /dl respectively. Mean value of serum uric acid were found 5.4 mg/dl and Plasma glucose (fasting) was found 100.3mg/dl in the given subjects. Conclusion: As bus drivers and conductors belong to lower socio economic status, thus free health checkup camps should be organized so that they can seek medical aid. Awareness must be created and Psychological counseling must be provided so that they adopt a healthy lifestyle and learn to cope up with the stress. Implementation of strict laws and rules are also advised so that safety of drivers and public is maintained.