Aim: The purpose of the present study was to assess the efficacy as well as pain perception status in patients, who have been administrated Inferior Alveolar Nerve Block (IANB) for third molar extraction by both closed mouth as well as open mouth techniques. Methodology: 90 patients were enrolled in the present study. The patients were given inferior alveolar nerve block to extract mandibular third molar by three different techniques. They were divided in 3 groups of 30 patients each in which conventional open mouth, Fischer 123 technique, closed mouth Vazirani Akinosi technique were assigned with Group I, II, III respectively. Pain Perception (VAS), Speed of anaesthesia, Patient anxiety and Clinician expertise were measured. Results: The results show that intra-oral conventional technique is less technique sensitive as compared to Vazirani-Akinosi and Fischer’s 123 techniques. Former is still the best in terms of less expertise required as well as pain perception by the patient. Conclusion: Conventional open mouth technique is still the most popular choice amongst clinicians and the pain sensitivity is also negligible amongst the patients with this classic intra-oral technique. Indirect as well as closed mouth technique requires more skilled expertise but can be used in cases with limited mouth opening.