In the moment it is on the market you can meet different types of impression materials differing in their properties and chemical composition. The purpose of the work was to test the cytotoxicity of various types of materials for taking impressions. Material and methods: Samples of 7 different materials (alginates, silicones, impression compound, zinc oxide eugenol, and acrylic) were tested on cell culture Vero CCL-81 in direct contact for a period of 2 hours. Results: One alginate material Elastic Cromo (71.35%) and Impression Compound (80.42%) haven’t negative influence on the cell cultures. Others significantly inhibit the development of cell cultures (Image 34.25%, Zetaplus 11.45%, Stomaflex Putty 8.02%, Repin 10.37%, FITT 28.92%). Conclusion: Most of the impression materials tested have cytotoxic properties.