A research was conducted to determine the occurrence and distribution of Escherichia coli (E. coli) O157:H7 in cattle faeces and manure, aimed at isolating and identifying shiga toxin producing E. coli (STEC) using phenotypic methods from cattle faeces and manure. Faecal samples (n=990) from apparently healthy cattle and manure samples (n=165) were collected between March to August, 2016 for this study, all samples were transported on ice to bacterial Zoonoses lab., department of Veterinary Public Health and preventive medicine Faculty of Veterinary Medicine, A.B.U. Zaria for analysis. Approximately 1 ml/1 g of faeces (homogenized when possible) were suspended into 9 ml of modified tryptone soya broth. Samples were vortexed and incubated overnight at 41°C. After selective enrichment, fifty micro liters of the product was streaked onto Eosin methylene blue (EMB), incubated at 37oC overnight. Preliminary results indicated 374/1155 (32.3%) of the samples showed greenish metallic sheen and were considered presumptive positive for E. coli and for further analysis. The 374 positive samples were tested biochemically using indole, methyl red, Voges Proskauer and citrate (IMViC), triple sugar iron and motility tests respectively, positive isolates 80/374 (4.7%) were subjected to Microbact 40E (U.K) where 48/80 (60%) were positive and were subjected to serological testing using E. coli O157:H7 agglutination test kits. The overall prevalence was determined to be 4.16% for all the samples collected in the three LGA, while a prevalence of 0.34%, 0.7% and 3.4% were found for Bauchi, Darazo and Katagum LGA respectively. Of the overall prevalence 2.51% were confirmed E. coli O157:H7 while 1.64% non O157. The prevalence based on sample locations were 0.09%, 3.37% and 0.69% for abattoir, cattle farms and livestock market respectively.