Medical students are inducted into clinical practices at a very early stage of their curriculum, and become very susceptible to get infected and for accidental transfer of infections to the patients, co- workers and the environment. Their awareness regarding Infection Control Practices (ICP) has to be regularly assessed by continued teaching and training programs. The aim was to assess the awareness of undergraduate medical students about infection control practices and measures and re-emphasize the need of continued education on basic infection control practices in the existing undergraduate curriculum. A cross-sectional study was conducted on 131 students of second year and third year (part I) MBBS of a Tertiary care teaching Hospital and Medical College. A pretested, predesigned questionnaire was given to the participants during their regular class hours after explaining the purpose of the study. It was designed based on the study objectives, that contained questions regarding general information of biomedical waste (BMW), hand hygiene, standard precautions, infection control practices and modes of acquisition of their knowledge. Amongst 131 students, 53.5% were females and 46.5% were males. The junior batch had a poor knowledge of the BMW responsibility (65.4%), use of masks (40.7%) and protocol for spillage management (32%). The senior batch had a better knowledge on moments of hand hygiene (90%) minimal time for hand hygiene (74%). The knowledge of senior batch on preventable vaccines (68%) and risk of transmission of viruses (76%) compared to junior batch (74% & 85.1% respectively) was poor. Most students acquired knowledge through lectures, tutorials and practical (74%) and suggested it to be continuously included in their curriculum through practical and workshops (65.6%). There was lacunae in knowledge regarding waste segregation, spillage management and use of masks. Continued education is needed through various methodologies. Importance of incorporation of ICP curriculum in undergraduate education should be re-emphasized.