To remove the inter-proximal plague, flossing has received the most attention among all the dentists. However, difficulty in dental flossing has made this technique to be avoided during tooth brushing. The proper transfer of Information and motivation to use dental floss and brush can change the attitude of the patient from an unhealthy approach to a healthy approach. The main aim of this study is to assess the knowledge and awareness of the adult population in Saudi Arabia about dental flossing. The pre-designed, pre-tested, and self-administered questionnaire survey was conducted among the adult patients visiting the various clinics in Saudi Arabia. The data that was collected from the questionnaire were analysed using the computer software Statistical package for social sciences that is SPSS 16. Among these 1011 patients, most of them were in the age group ranging from 19 to 25 years. About 971 (55%) participants used toothbrush and toothpaste to clean their teeth, while 234 (23%) patients used mouthwash, and 177 (17%) used miswak. Only 140 (14%) participants used the dental floss, 566 (56%) didn't use dental floss, and 305 (30%) rarely use dental floss.