Aim: To investigate the potential capacity of limbal mesenchymal stem cells (MSCs) incorporated in fibrin glue as cell delivery system in modulating wound healing after trabeculectomy. Methods: Limbal MSCs were obtained from rabbit corneoscleral tissue. MSCs adhesion on fibrin glue derived from the mixture of fibrinogen and thrombin in concentration 1:1 and 1: 0.5 were observed 2 hours after cells seeding. Cell proliferation was assayed by modified tetrazolium method (MTT assay) on day 3. Cells adhesion and viability were analyzed using independent t test (SPSS 19 version). Preliminary study in animal model was conducted in 6 rabbit eyes to observe the role of fibrin glue as cell delivery system. MSCs were labelled using PKH26 prior to subconjunctival transplantation following common trabeculectomy procedure on rabbit eyes. Two eyes were enucleated on day 7, 14, and 21 to obtain conjunctival tissue of trabeculectomy site. Frozen sectioned specimen of conjunctival tissue was observed under fluorescence microscope to analyze cells engraftment and survival. Results: Isolated cells from corneoscleral tissue showed MSCs as they were positive for CD73,CD90,CD105, and negative for CD45. There were no significant differences of cells adhesion (p=0.3) and viability (p=0.2) between fibrin glue composed of fibrinogen:thrombin 1:1 and 1:0.5. Cells engraftment and survival were observed during experimental periods on day 7,14, and 21. Cells began to migrate on day 21 as the time of fibrin glue degradation. Combined MSCs and fibrin glue may facilitate wound healing modulation after trabeculectomy. MSCs may release antifibrosis factors slowly as gradual degradation of fibrin glue. Moreover, fibrin glue properties may promote cells engraftment and survival. Conclusion: Combination of fibrin glue and MSCs may be an alternative for modulating wound healing after trabeculectomy