The aim of the study was to investigate Alkaline phosphatase (ALP) activity in peri implant crevicular fluid (PMICF) in direct and indirect anchorage side during orthodontic tooth movement in humans. Material and Method: A split mouth technique of ten patients requiring all first premolar extractions were selected and treated with absolute anchorage. Enmase-retraction was done using 150 g sentalloy springs at both direct and indirect anchorage side. Maxillary Ist quadrant side acted as direct anchorage site, while second quadrant acted as indirect anchorage site. Peri implant crevicular fluid was collected from mesial crevices of mini implant before initiation of retraction (baseline), and after initiation of retraction on 1st, 7th, 14th and 21st day and the Alkaline phosphatase activity was estimated using ELISA method. Results: There was no significant difference in means value of Alkaline Phosphatase of two groups at any interval. But the mean difference of Alkaline Phosphatase for all intervals between two group was statistically significant. Conclusions: The overall means of Alkaline Phosphatase of direct method was significantly (p < 0.05) higher than indirect anchorage group.