Background: Removal of endodontic infection is quite different from most other sites in the human body. Aim: To investigate the antibacterial property of biodentine and mineral trioxide aggregate cement against Streptococcus and Enterococcus. Methods: Antibacterial activity of Biodentine and mineral trioxide aggregate was evaluated by the agar diffusion method against E. faecalis and S. mutans among sixty patients. The experimental materials included 50 mg Biodentine and 50 mg mineral trioxide aggregate. The diameter of microbial inhibition zones around each well was measured to the closest size in mm with a digital caliper. Results: Biodentine produced 3.02±0.27 mm inhibition zone against Streptococcus mutans and Enterococcus faecalis within 24 hours. This difference between the S. mutans and E. faecalis was not statistically significant. Sililarly Mineral trioxide aggregate produced 2.01±0.22 mm inhibition zone against Streptococcus mutans and Enterococcus faecalis within 24 hours. Conclusion: Biodentine showed us encouraging results against Streptococcus and Enterococcus compared to mineral trioxide aggregate by creating higher inhibition zones.