Certain physiological changes occur during normal pregnancy. These changes when exaggerated could result to adverse maternal outcome. Adverse cardiovascular outcomes have been previously linked with hypoalbuminemia in the general population. Few reports exist about serum albumin level in second and third trimester of pregnancy. Our study aims at determining serum albumin level in second and third trimesters of pregnancy. This was a case-control study involving randomly selected 40 non pregnant and 77 pregnant women in Makurdi, Nigeria. The participants were subdivided into 3 comparable groups; control n=40, second trimester n=37 and third trimester n=40. Mean age, body mass index (BMI), and serum albumin in the study groups were compared using ANOVA. Associations of age, BMI, gestation age and albumin were determined using Pearson correlation analysis. Serum albumin level was significantly (P<0.0004) lower in the pregnant groups compared to the controls. A post hoc test reveals a significant (P<0.0001) lower serum albumin in third trimester group than in second trimester and controls. Conclusion: Knowledge about hypoalbuminemia during pregnancy will help distinguish pathological hypoalbuminemia during antenatal checkup.