Background: Individuals should spend at least 2 minutes brushing their teeth with an effective technique at least twice a day, However, most estimates of actual brushing time vary between just over 30 seconds to 60 seconds. Aim: To study the relationship between time of manual tooth-brushing and plaque removal. Methods: Turesky index was used in this study group to assess the plaques. Brushing times were assigned over a 3 week period and brushing times assigned were 30, 45, 60, 120 and 180 seconds. Dental plaque before and after brushing were evaluated and recorded. Results: Changes in Turesky index score for 30 seconds brushing from pre brushing to post brushing were 3.276 ± 0.064 to 2.628 ± 0.175. For 45 seconds brushing time, pre brushing and post brushing scores were 3.262 ± 0.036 and 2.648 ± 0.151. Turesky index score for 60 seconds changed from 3.287 ± 0.065 to 2.562 ± 0.114. for 3 minutes brushing, pre brushing and post brushing scores were 3.064 ± 0.071 and 2.657 ± 0.143. Conclusion: Oral health education towards educating the patients about the duration of brushing time required for plaque removal.