Introduction: Identification of human being is always a problem for scientists and is based upon scientific principles. Finger prints, dental data, anthropometry & DNA analysis are the tools used for identification purposes. The new arriving tool in the field of personal identity is cheiloscopy i.e. study of lip prints which are unique for every individual and behold the potential for identification purpose. If lip prints are found at scene of crime, the presence or absence of a person can be ruled out from the scene of crime. Aim: The objective of the study was to check for any peculiar lip patterns in relation to the sex of the individual and determine the most common lip patterns by using Suzuki &Tsushihashi’s classification. Materials and methods: A study group of 100 undergraduate students (50 males and 50 females) in the age group 18-23 years were chosen randomly from our institute. Non-glossed lip stick color, white bond paper, cellophane tape, scissors, and magnifying lens were used to obtain lip prints and analyzed with the help of a personal computer and Adobe photoshop software. The lip print pattern of middle part of lower lip was considered, as it is visible in almost all the prints. Result: Majority of the study group (28 people -28%) belonged to Type IV and 6 people (6%) belonged to Type V group. The most common pattern found in female was II, while type IV was predominant among males. Conclusion: Studies show lip prints have great potential to establish individuality, show gender variation and remain unchanged forever.