Occupational health and safety in the health sector has gained more importance over recent years. Education and work in hospitals that are involved in the 'very dangerous workplaces' class are valuable in supporting prevention of workplace accidents and occupational diseases. The aim of the study is to determine knowledge status of non-physician health personnel, to receive their opinions about their work and working environment, and to evaluate the occupational health and safety education within the context of the "Occupational Health and Safety". The study was conducted with non-physician employees in a university hospital. It was considered sufficient to reach 80% of the employees without using any sampling method. A questionnaire form was prepared as a data collection tool. Before the training, the questionnaire and the 'Occupational Safety Scale for Hospital Health Care Personnel' were completed. Interactive training was provided by the researchers later on. After the training was completed, a final test was conducted with 26 questions in the preliminary test. Descriptive statistics and tests appropriate for distribution of the variables were used to analyze the data. The limit value for significance was accepted as p <0.05. 61.7% of the participants were nurses and 38.3% were other hospital employees.