Introduction: Improper handling and disposal of medical waste is hazardous to waste handlers, health care workers, environment and also increases the risk of nosocomail infections. This study was planned to evaluate the practical calibration and awareness of dental auxillaries in disposal of hazardous biomedical waste generated during dental treatment into color coded dustbins at a dental hospital. Material and Methods: The study comprises of 18 dental auxillaries who was asked to dispose the simulated biological, non-biological and semibiological material according to their knowledge into the color coded dustbin. The study was planned and carried out three times (for each dental auxillary) at different days. As each respondent was asked to dispose the waste of each category three times, thus waste was disposed for 162 times. Results: were expressed as a number and percentage of respondents for each question and were analyzed using the SPSS Version 10 software. Chi-square test was performed and the level of significance was set at p < 0.05. Results: The correct disposal of biological waste in red coded dustbin was n=31 (57%), non-biological waste in green coded dustbin was n=30 (54%), semi biological waste in yellow coded dustbin was n=27 (50%). Thus waste was correctly disposed 88 times (54%). Conclusion: Dental auxillaries should be motivated to attend training and education programmes concerning waste management so that they will be efficient to properly segregate, disinfect and dispose hospital waste in an eco-friendly way.