The study compared the knowledge and practice of dental students regarding infection control policies in College of Dentistry, Taibah University, KSA. Self-administrated questionnaire were applied to dental students of 3rd, 4th and 5th year (n = 51). Chi-square test were used for statistical analysis of data. Significance level was set at p < 0.05. The response rate was 100%. Knowledge of the students regarding infection control policies, none of the respondent obtained a full score of 13 (highest knowledge). The lowest score obtained (15) and 16% of respondent obtained this score. The highest score was 23 (poor knowledge). (57%) of third year students had an acceptable score of less than 18. The best score for fifth year students was 15 (29.4%) and the worst score of 21 was found in two students. The knowledge of students in relation to their year of study showed no significant differences (P > 0.05). Almost 70% of respondents did not know the correct procedures to follow after having a needle stick injury. There were no sig- nificant differences (P > 0.05) between the student’s knowledge regarding the procedure to follow after a needle stick injury and the year of study. After comparing the knowledge of students to their observed practices in the clinics, Two thirds of the students reported not to wash their hands before and after each patient. (67.6%) of students don’t wash their hands before the procedures and (15.8%) of students don’t wash their hands after treating patient. More than 90% of respondents who were observed did not wear goggles whilst treating patients.