This study aimed to determine the prevalence and risk factors of the neck and back pain among students and staff of the Dental centre, University of Port Harcourt Teaching Hospital (UPTH). This study was conducted among one hundred subjects, comprising of ten consultants, eight residents, fourteen house officers, fortythree dental students, fifteen dental surgery assistants, four therapists and six technologists. Evaluation of the subjects with self-administered questionnaires was done, which comprised subjects’ biodata, posture during work, rest period at work, exercise during rest and location of pain. Pain severity was assessed using the Visual Analogue Scale (VAS) (0 -10) pain scores. Fifty-eight percent of the participants were female, with male to female ratio of 1:1.34 and mean age was 30.23±8.49years. Seventy-six percent (76%) had experienced back pain with neck pain predominating in 38.2% followed by upper back pain (31.6%). There was no statistical difference between the percentage of males (76.2%) and females (75.9%) that have experienced musculoskeletal pain. According to the cadre of the respondents; 100% of residents, 90% of consultants, 86.7% of DSA and 83.3% of dental technologist have experienced musculoskeletal pain in their practice. Only 14.5% of the respondents had missed practice due to pain. Majority (49.0%) compromised their posture while working and only 9% do any form of exercise during rest period. There is a high prevalence of neck and back pain among dental professionals in this study. Years of practice, number of patients seen per day and posture plays a vital role. Hence, a review of the practice of dentistry is needed.