Wounds are a significant cause of morbidity worldwide, studies show that for every million wound patients, at least 10.000 die from microbial infections. The skin is a vital organ that serves as a protective barrier between the human bodies on its external environment. Staphylococcus aureus has been known as a cause of deepseated wound infection for close to a century, having recognized as a cause of nosocomial infection and super infection in patients receiving antimicrobial agent such as surgical cases. MRSA colonizing the anterior nares and skin of human are the major sources of surgical site infection as well as nosocomial spread. Methicillin-resistant Staphylococcus aureus (MRSA) are bacteria resistant to penicillin and cephalosporin classes of antibiotics and are often resistant to many other classes of antibiotics. Importance of S. aureus as persistent nosocomial and community acquired pathogen has become a global health concern. The aim of this study to determine the frequency and antibiotic susceptibility pattern of S.aureus isolated from wound infected patient attending Wad Madani Teaching Hospitals. Two hundred samples collected from patients with infected wound at the period from April to July 2016. Out of 200 samples examined, 60 (30%) isolates of S. aureus obtained, 116 (58%) other bacterial species and 24 (12%) samples showed no growth. The highest isolation rate of S. aureus 60% occurred among age group (41-60) and according to gender 32 (53.3%) S. aureus isolated from male and 28 (46.7%) from female. The overall profile of susceptibility pattern showed that vancomycin and linezolid were more sensitive (100%), amoxicillin and penicillin were the least sensitive (10%) and (36%) respectively. The result of the study showed that frequency of S. aureus isolated from infected wounds 60 (30%) and vancomycin and linezolid are more effective in the management of S. aureus in this locality.