H pylori infection is well known to be the most common human infection worldwide on the basis of the fact that approximately 50% of the worldwide populations are infected and that human beings are the main reservoir. Infection is more prevalent in developing countries, and incidence is decreasing in Western countries. Moreover, it has been proved to be associated to endocrine system regulation of various hormones in the body, prolactin (PRL) is one of the reproductive hormones, which is secreted by the anterior pituitary gland. The biological actions of prolactin hormone are Lactotrophic effects, metabolic effects influence to action of other hormones, osmoregulatory effects, and Influence of prolactin on the immunological system. This study was conducted in Khartoum State at the period from December 2015 to May 2016, and aimed to determine the possible effect of H. pylori infection on serum prolactin levels in Child-bearing females age (15 – 45). Sample size was 50 females infected with H. pylori and 50 uninfected females as control group. Stool Samples were taken from the entire study subject (100) samples to confirm the results of H. pylori Ab ICT in serum. The stool samples were analyzed by using H.pylori stool Ags detections ICT. Serum prolactin level was measured by using Cobas e411chemistry analyzer, the results showed that Prolactin concentration was higher in the patients compared with controls. In this study hyperprolactinemia due to H.pylori infection was reported in 52% of the patients. Statistical analysis showed that there was significant difference in the mean of prolactin concentration between infected and uninfected females group (P= 0.000). Regarding correlation between prolactin concentration and subject’s age, H. pylori infection duration or recurrence of infection, no significant was found to be observed.