Vitamin D plays a role with hormones in their receptor sites. Without vitamin D, hormones cannot function the way they would normally. The receptor sites malfunction, leaving estrogen, progesterone, testosterone, luteinizing hormone, and follicle stimulating hormone in the lurch. Without healthy action at receptor sites, hormones don’t get utilized. The menstrual cycle fails as a result PCOS results. There is no published data about the relationship between vitamin D deficiency and polycystic ovaries syndrome among Sudanese ladies with infertility disorders. To know the association between vitamin D deficiency and polycystic ovaries syndrome among Sudanese ladies with infertility disorders. Descriptive, cross-sectional study, 39 Sudanese ladies with known PCOS and infertility disorders were involved in the study. 71.8% of ladies with polycystic ovaries syndrome were vitamin D deficient. Further studies must be carried out, using large sample size and more than one diagnostic technique.