Urinary tract infection (UTI) is one of the most common infectious diseases globally. An increasing antimicrobial resistance among Community-acquired (CA) and Nosocomial UTI has been rapidly growing. Cross-sectional, analytical and descriptive study was conducted to investigate the current in vitro susceptibility pattern of the clinically isolated bacteria among Community and Hospital-acquired (HA) UTI. Patient data for 421 urine specimens were collected from the case records during October 2016 to March 2017. Significant growth was yielded from 170 of urine specimens. Kirby-Bauer disk diffusion method was used for detection antibiotic susceptibility pattern for all isolates. Of the total 170 (40.4%) culture positive samples, 51.2% and 48.8% were CA and HA UTI respectively. The culture positivity of UTI among the female was (44.1%) compared to (55.9%) among the male. There was a significant difference in resistance pattern between CA and Nosocomial UTI. The distribution of the isolated pathogens were (51.2%) and (48.8%) in CA and HA respectively. The predominant bacterial isolate was Escherichia coli (43.5%) in both cases of community and nosocomial UTI. However, Klebsiella pneumoniae (69.0%) was mostly isolated from HA-UTI while Staphylococcus aureus (80.0%) was mostly detected in CA-UTI. The recent study revealed high resistant rates of UTI among HA as compared to CA. This study revealed that more than 90% of HA-UTIs isolated pathogens were resistant to broad-spectrum Penicillins, Cephalosporins, Quinolones, and Macrolides. Contrarily, high rates of bacterial sensitivity was found towards Imipenem (82.9%), Nitrofurantoin (72.4%), Ertapenem (70.7%), and Amikacin (67.1%). Even if Escherichia coli remains the major pathogen in UTI, the resistance pattern of CA-UTI is frightening in our country. Nitrofurantoin is the only oral drug that retained reasonable activity against most UTI pathogens. Imipenem and Ertapenem remain effective for UTI bacterial isolates. The changing of UTI etiological agents was observed in the current study.