Periodontal disease has been recently linked to a variety of systemic conditions such as diabetes, cardiovascular disease, preterm delivery, and oral cancer. Oral Squamous Cell Carcinoma is a lethal disease whose incidence is increasing. The most common bacteria associated with periodontal disease, Porphyromonas gingivalis (P. gingivalis) has not yet been significantly studied in the malignant gingival tissues. The objective of this study was to investigate the presence of P. gingivalis in specimens taken from the deeper tissue biopsy in squamous cell carcinoma patients. We have performed Real Time Polymerase Chain Reaction(PCR) technique to investigate the presence of P. gingivalis in deeper tissue biopsy samples of gingival squamous cell carcinoma. P. gingivalis is abundantly present in malignant oral epithelium suggesting a potential association of the bacteria with gingival squamous cell carcinoma. These observations may help to explain, in part, the predominant role that P. gingivalis plays as an infectious agentin oral malignant patients.