Tobacco use is a serious public health problem, the prevention and control of tobacco use has become an emerging issue of global significance and of central importance to oral health and Dental care. So, health professionals have a critical role in reducing the tobacco use in which dentists play a significant role. Thus, this study was conducted to assess and compare the Knowledge, attitude and perception towards tobacco use among dental and engineering students. A self-administered structured closed ended questionnaire to 440 Undergraduates was used. The chi-squared test was used to check differences between proportions. The unpaired t-test was used to check mean differences between the groups. Majority of the students in both the group had a fair level of knowledge related to tobacco regulations. Mean knowledge score between both the groups towards ill-effects of tobacco showed that dental students had good knowledge level as compared to engineering students (P=0.000). 145 (94.8%) dental students and 243 (84.4%) engineering students expressed a positive attitude towards advising smokers to stop smoking. Among dental students, 129(84.3%) perceive that attractive packaging of tobacco products is bad as compared to 181(62.8%) engineering students. Although attitude and perception among dental students towards tobacco cessation was better than engineering students, knowledge of tobacco regulations in both the groups was not satisfactory.