Alkaline Phosphatase comprises a group of enzymes that catalyze the hydrolysis of phosphate esters in an alkaline environment. This enzyme plays an important role in the bone metabolism. Alkaline Phosphatase acts as a byproduct and its serum level directly corresponds to the osteoblastic activity. The aim of the present study was to measure the serum and salivary alkaline phosphatase levels to detect the rate of bone resorption. In the present clinical study, serum and saliva samples were collected from the female patients between the age groups of 30-70 years and subjected for the biochemical analysis of alkaline phosphatase and calcium in semi-auto analyzer and were analyzed statistically. Serum and salivary alkaline phosphatase and calcium levels were decreased gradually with progressive age. By the present study, we concluded that levels of alkaline phosphatase and calcium will decrease with advancing age and saliva can be used as a diagnostic tool alternate to serum.