The major objective of the present study was to find out the familial alienation of transgender with reference to District Swat-Pakistan. A sample size of 210 respondents was selected from total population 400 as per Sekeran criteria. The data was collected through interview schedule and analyzed by using descriptive statistics frequency and percentage) and inferential statistics (Chi-square test) to find out the association between transgender (Dependent variable) and familial alienation (Independent variables). The results showed that a highly significant association (P=0.000)was found between “Transgender” & “Familial Alienation” indicators; transgender often deal with gender disparity; transgender faced physical and psychological violence from family members; family members do not permit transgender to meet with other transgender along with transgender were killed in the name of honor. The study concluded that, family as an institution was found in total disarray with reference to performing its basic role pertaining to transgender. A comprehensive plan of action based on protecting them by introducing laws pertaining to their protection at macro and micro level specifically at family level is the order of the day were put forwarded some of the recommendations in the light of present study.