The role of university today is to greatly contribute in the development of Start-Up Business in accordance with its capacity as an intellectual source through the formation of the students’ atmosphere into a reliable entrepreneur in the future. The strategy is to create an entrepreneurial atmosphere in the university through a business and technology incubation model development that focuses on start-up business based on the creative economy sub-sector. The purpose of this research is to strengthen the incubation institute of the business and technology in the university with the approach of model and method of work, the development of business incubation program, and also to produce tenants of start-up business in the university. The result of this research found that there is effective model of business start-up development in university through an ideal and effective business incubation that is replicated and implemented by other universities. The next finding in establishing an entrepreneurial atmosphere model is through a start-up competition program among universities.