The impact of mining activities on environmental and human health has become a matter of serious concern. Human health and vegetation is affected by decline in the biological, physical, and chemical quality of the environment. On-site pollution in coal mines due to dust, gases, noise, and polluted water is receiving increasing attention because it is affecting coal miners’ health as well as those living in close proximity. Toxic pollutants released in vicinity by such processes include lead, nickel, chromium and cadmium. Focus of research was on the fate of toxic heavy metals in mining areas to evaluate and compare the heavy metal status in directly exposed persons (coal miners) of two different mining regions of Punjab (Pakistan). High significant difference was observed for cadmium, lead, chromium and nickel in nail and serum samples of coal miners. Comparison of metal concentration in biological samples of chakwal and soon valley area shows that soon valley coal mine area is more polluted than chakwal coal mine area.