Innovation and entrepreneurship is the new engine of national economic development. Non-profit organizations are an important force in promoting the development of innovation and entrepreneurship. Based on the specific practice of Enactus, one of the three largest college student organizations in the world, exploring the path and experience of non-profit organizations participating in the innovation and entrepreneurship of college students in China is conducive to the formation of the pattern of multi-agent participation in the cultivation of innovative entrepreneurial talents, and further promote the effective development of college students' innovation and entrepreneurship education.