The Scarlet Letter has aroused people‟s attention since its publication. The analysis of the protagonist, Hester Prynne in the novel, has always been a heated topic at home and abroad. Scholars have interpreted Hester Prynne from many perspectives like Archetypal Criticism, Modernism or Feminism, especially eco-feminism. Therefore, this article tries to extend the previous‟ studies in terms of Androgyny. Based on the theory of Androgyny, this article aims to analyze both the femininity and masculinity of Hester Prynne, which is to reveal the deconstruction of binary opposition in the traditional viewpoint of sexuality. Hester Prynne's femininity can be interpreted from her feminine charm and maternity, while her masculinity can be analyzed from rebellious spirit and survivability. Besides, this article explores the influence of Hester's androgynous characteristics on herself, Dimmesdale, Chillingworth and other people around her, which is to embody Hester's self-growth. As a result of her innermost androgyny, Hester grows from an unworldly young woman to a strong-willed, independent and mature woman.