Despite numerous studies have placed the emphasis on an examination of individual differences in humor styles, the investigation of this topic in Thai context has been overlooked and needs to be explored. As a consequence, the objective of this present study was to examine individual differences including gender, age, and body mass index (BMI) in humor style of Thai managers in real estate companies. Seventy-nine managers in all hierarchical levels of two real estate firms listed in Stock Exchange of Thailand were gathered for data collection by using Humor Style Questionnaire (HSQ) as the instrument. The result of independent sample t-test showed that male managers had a higher score on the use of self-defeating humor style than female managers (t = 2.806, p = 0.007). In addition, the result of KruskalWallis H test demonstrated that there was a statistically significant difference in selfenhancing humor between the different age groups (χ2 (3) = 8.731, p = .033). Nonetheless, results of Kruskal-Wallis H test indicated no statistically significant differences in all four humor styles between the different BMI groups. Discussion and recommendations for further studies were also discussed.