Kinetics of biodiesel production from renewable raw material like soybean oil deodoriser distillate (SBODD) and methanol (MeOH) in the presence of biocatalyst Novozyme 40013 (lipase from Candida antarctica) has been studied in the present research investigation along with optimization of reaction parameters like molar ratio of alcohol to SBODD, temperature and catalyst concentration. 5:1 molar ratios of alcohol to SBODD, 600C temperature with 5% concentration of catalyst for 3 hrs of reaction duration are the optimum conditions identified which contributed 93.24% conversion. Pseudo homogeneous kinetic model has been used to fit the experimental data and results have been analysed on the basis of model data. Equilibrium constants (Kobs) have been evaluated through this kinetic model at different temperatures and different molar ratios. By fitting the experimental data, the pre exponential factor and the activation energy of the esterification reaction have been determined also. Kinetic analyses are in good agreement with experimental results.