In vitro embryogenesis and plant regeneration were studied in date palm cultivar, Khalas using three osmotic agents (PEG, Mannitol, and Glucose) and NaCl for five different time periods ranging from 1 to 24 hrs. The highest frequency of somatic embryogenesis was recorded in the medium containing 15% PEG in the MS-culture medium. Similarly, the highest number of plantlets was also obtained on the regenerating medium containing PEG under elevated treatment time periods. Mannitol and glucose showed almost the same response at all treatment levels producing on an average 2 to 3 embryos per culture but did not had any positive effect on plant regeneration. Of all the osmtic agents, PEG proved to be better in producing more callus fresh and dry weights followed by mannitol indicating that at this stage these osmotica worked as non-metabolic osmotic agents. The use of NaCl as an osmotic agent produced the most adverse effects on callus fresh weight and embryogenesis, with no embryos formation at 12 and 24 h treatments. These studies revealed that elevated levels of PEG showed stimulating effects and helped in the production of more callus mass, somatic embryos and plantlets formation compared to other osmotic agents used.