Bio-fuels are the most promising renewable energy which may provide a viable mode of alternative system and can be a real substitute to fossil fuels. Nevertheless, this technology ought to overcome a number of hurdles before it can compete in the fuel market and can be broadly organized. Consequently, the present research was attempted to evaluate the esterified oil obtained from „Gingili (Sesame) seed‟ (botanically called Sesamum indicum, L. belongs to family Pedaliaceae) for various physico-chemical parameters followed by performance of diesel engine with variable blends. Accordingly, the oil generated from the seeds of Gingili was subjected for transesterification with methanol in presence of catalyst NaOH. The fuel properties of Gingili (Sesame) bio-diesel can meet up with the specification of ASTM such as, Color (pale brown), Specific Gravity at 150 C0.88, Kinematic viscosity@400C 5.22, Cetane number 53.20, Sulphur, wt% 0.0083, Cloud point 12, Pour point -10, Lower Heating Value (KJ/Kg) 40676.848, Flash point 2400C, Fire point 1840C. Further, an imperative performance of diesel engine was recorded with increase in ratio of fuel blends of 10%, 20%, 30%, 40% & 50% respectively. In addition, the comparative analysis was also carried-out between both Gingili (Sesame) bio-diesel and petroleum diesel with diesel engine system at variable loads and the performance characteristics of the engine were analyzed. The engine test results showed significant thermal efficiency in Gingili biodiesel compared to petroleum diesel with respect to performance and emission parameters. The emission parameters like, Carbon monoxide (CO), Oxides of Nitrogen (NOx), hydrocarbons (HC) was found to be low when compared to petroleum diesel. In conclusion, Gingili biodiesel found to be most significant source as compared to petroleum diesel on the basis of over all performance and emission parameters deliberated via analysis.