Asparagus racemosus Willd belongs to the plant family Asparagaceae and one of the well-known medicinal plants used in indigenous systems of medicine of Sri Lanka. It has abundant medicinal effects such as aphrodisiac, antioxidant, immunostimulant, antihepatotoxic, antibacterial, antidiabetic, anticarcinogenic, antidiarrheal, antiulcerogenic, antioxytocic, etc. Local suppliers for A. racemosus cannot meet the country requirement and therefore, mainly depends on imported A. racemosus grown in India. Therefore, an attempt was taken to evaluate the physico-chemical, phytochemical and nutritional profile of A. racemosus grown in India. Results revealed that 6.2 0.1% of total ash, 1.9 0.0% of water-soluble ash and 1.7 0.0% of acid insoluble ash were contained in roots of A. racemosus grown in India. Total phenolic content, total flavonoid content and total saponin content were 14.0 ± 0.1 mg gallic acid equivalents/g of extract 7.1 ± 0.2 mg quercetin equivalents/g of extract and 4.5±0.2 % respectively for A. racemosus grown in India. Twelve prominent spots bearing Rf values of 0.07, 0.09, 0.12, 0.20, 0.25, 0.28, 0.36, 0.48, 0.54, 0.71, 0.89 and 0.93 were present in Indian origin A. racemosus. Heavy metals such as Hg, As, Cd and Pb and microorganisms including Staphylococcus aureus, Escherichia coli, Coliforms, Salmonella and yeast and moulds were not detected in A. racemosus roots. Energy value of A. racemosus grown in India was 22 kcal/100g. In conclusion, present study illustrates a clear idea of physico-chemical, phytochemical and nutritional properties of A. racemosus grown in India.