Natural products, such as plants extract, either as pure compounds or as standardized extracts, provide unlimited opportunities for new drug discoveries. The research is aimed to investigate the physicochemical properties and characterization of Eucalyptus camaldulensis leaves oil. The physicochemical were evaluated using the standard procedure and the oil was fund to be light yellow color having camphor like smell. The pH of the oil at 22oC was found to be 4.62 while the % oil yield, Optical activity, Refractive index at 27°C, Specific gravity at 27°C, were found to be 49.5±1.021, +34.2°, 1.4384 and 0.812 respectively. The absolute and kinematic viscosity of the leaves oil were 102.30 and 88.20 respectively and the Total acid number (TAN) in mg KOH/g, Iodine number and saponification number were 1.67, 109 and 110.00 respectively. GC-MS revealed the presence of 16 compounds representing 86.96 % of the total oil. The major component was 1,8-cineole (49.08 %), Eucalyptol (34.42) followed by α-pinene (23.90 %), while L-pinocarveol (8.98 %) and globulol (2.71) were the least components. Also α-terpineol, myrtenol, camphene and ciscarveol were present in the oil. The result revealed that Eucalyptus camaldulensis leave oil consist mostly of oxygenated monoterpenes that could be very useful for human, animals and industries, also the oil met the FOA/WHO standard of oils. Therefore, these essential oils could be considered as promising substances for development of new drugs. Hence it potential for medicinal and pharmacological uses.