Background: Infectious diseases are the leading cause of death world-wide despite the vigorous campaigns that have been made to combat them. This has been occasioned by drastic growth of drug resistant pathogens. Plant based antimicrobials represent a vast untapped source of medicines and a further exploration of plant antimicrobials is called for. Plant extracts have led to the discovery of many clinically useful drugs such as emetine, berberines and quinine. There is a continuous and urgent need to discover new antimicrobial compounds with diverse chemical structures and novel mechanisms of action for new and re-emerging infectious diseases. Therefore, researchers are increasingly turning their attention to exploring untapped opportunities of natural products, looking for new leads to develop better drugs against microbial infections and other diseases. The objective of the study is to determine In-vitro antibacterial activity of different extracts and fractions of Lophira lanceolata plant. In this study, the plant extracts were screened for their antibacterial activity against selected strains of bacteria, including Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Bacillus subtilis and Staphycoccus aureus). The diameter zone of inhibition was determined using agar well diffusion method. Ethanol extract was found to have the highest activity while aqueous extract has the least against the tested strains. The n-hexane, ethylacetate and residual aqueous fraction have no activity whereas the n-butanol fraction has activity. The MIC of ethanol extract was found to be inhibitorier than methanol and aqueous extracts. The MBC of the extracts revealed that methanol, ethanol and aqueous extracts are bactericidal on one or more organisms while n-butanol fraction is bacteriostatic to all test strains at the concentration used. Conclusion: Conclusively, the stem-bark of Lophira lanceolata possessed in-vitro antibacterial activity with highest active observed in ethanol extract.