Background: Nurses are the major work force of any health care. Highest incidence rates of work-related low back problems seen among nurses. Thus the present study was conducted with the aim to determine effect of conventional therapeutic exercise on low back pain. Methods: Quasi experimental research design was adopted with 60 samples who met the inclusion criteria. Samples were allocated into experimental group (n=30) and control group (n=30) by convenience sampling technique. Pre-test was conducted by using Numerical pain rating scale and Rolland Morris Low Back Pain and Disability Questionnaire in both experimental and control group. Conventional therapeutic exercise was administered to the experimental group and the control group was continued with the routine procedure. Post-test was conducted after 2 weeks with same tool for both the group. Data were analyzed using SPSS. Results: The study findings revealed that the low back pain and its related disability was present among staff nurses working in intensive care unit and operation theatre. Conventional therapeutic exercise was effective in reducing the level of low back pain at the level of p<0.001 in the experimental group. Conclusion: Conventional therapeutic exercise may be routinely practiced in the hospital thereby preventing the low back pain which in turn makes the nurses efficient to provide productive and quality nursing care.