Background: Occupational safety and health is concerned with protecting the health, safety and welfare of people engaged in work or employment. It is a duty of every employer to ensure that all its employees remain safe at all times in order to boast its production activities. Objective: The study assessed the supply, use of personal protective equipment and factors that may affect its usage. Methods: The study is a descriptive cross-sectional study. There were 250 respondents from registered medium scale manufacturing industries in Anambra State. The sample was selected using a multistage sampling technique. The number of respondents from each factory was allocated proportionately according to size of the individual factors. Data was collected from October 2013 to May 2014. A pretested interviewer administered question was used. Analysis was done using statistical packages for social sciences version 20. Level of significance was set at 5%. Result: The supply of personal protective equipment was low as only hand gloves was always supplied (100%) while face mask and ear plug was never supplied. Use of personal protective equipment was assessed as never used, sometimes used or always used. More respondents have never used most of the personal protective equipment as 83.2%, 21%, 70.7%, 77%, 77.7%, 79.9%, 73.2% have never used helmets, hand gloves, goggles, safety boots, face marks, ear plug and apron respectively. The workers reported inadequate knowledge of use of personal protective equipment (42.4%) and unavailability of equipment as the major reason for poor use of personal protective equipment. Conclusion: The poor use of personal protective equipment, result from poor knowledge of its applicability and lack of the personal protective equipment. Recommendation: Government and relevant agencies should set up laws and enforce the supply of personal protective equipment by management of factories. Factories should employ a factory inspector who will enforce the compulsory use and also educate employees on the use and benefits of usage.