In the Lubumbashi plain, the dominant soils are acidic Ferralsol that contain small amount of available phosphorus and nitrogen. The organic matter content is also limited. This study evaluated the effect of Arbuscular mycorrhizal fungi (AMF) on root colonization in Phaseolus beans growth performance and yield. Three soils with a pH 5.8; 6.2 and 6.5 respectively were included. We also studied the effect of increasing doses of P2O5 in beans by providing 25kg of P2O5/ha, 50kg P2O5 ha-1 in combination with AMF inoculation. The results show a high frequency of colonization at a pH of 5.8 (69%); a plant height of 56 to 58 cm with 10 leaves and 6 pods per plant for a yield of 2095kg ha-1. However, above pH 6.2, root colonization is low (31 to 61%) and plant height ranges from 27 to 39 cm with 4 to 6 pods for a yield between 631 and 1479 kg ha-1. The effect on plant response of 25kg P2O5 ha-1 in combination with AMF inoculation was statistically significant for all measured parameters. Finally, this study compared the efficacy of inoculum produced on three different way on colonisation efficiency of beans. Inoculum produced by using Plantago lanceolata as host species contained more spores and resulted in better-colonized roots compared to inoculum coming from Sorghum vulgare or clay inoculum.