The production of bananas and plantains is currently practiced on a large scale due to the high demand on the market, as a result of the strong demographic pressure in large cities. There is currently a lot of research being done on the use of symbiotic systems associating plant species with mycorrhizal fungi. Mycorrhizae give host plants the ability to develop in mineral-poor soils. As mycorrhizae are not well known in our environments and few studies have been carried out on their biodiversity, macroscopic and microscopic counts and characterizations, this study seeks to identify and characterize these mycorrhizal strains in symbiosis with the plantains of the Simi-simi experimental site at the University of Kisangani and then inoculate them with vitro plants from the plantains of two cultivars from in vitro culture. The root staining technique after four months of cultivation detected mycorrhizae in the roots of these two plantain cultivars. The results of this study revealed that the overall degree of mycorrhization is 80% on the total observed roots and phenotypic characterization has grouped the spores into four genera which are: Glomus, Acaulospora, Scutellospora, Gigaspora and a group of uncharacterized spores.