Background: Improvement in ultrasonography over the years have allowed visualization characterization of fetal morphology. Objective: The study was aimed at determining the awareness and acceptability of ultrasound gender determination among women attending the antenatal care clinic of the Usmanu Danfodiyo university teaching hospital Sokoto. Materials and Methods: This study was a cross sectional study where pregnant women were selected by simple random sampling. The data was collected using pretested interviewer administered questionnaire. Relevant information was obtained. A P value <0.005 was considered statistically significant. Results: Among the 200 women recruited, (57%) were within the age group of 20-29 years with a mean age of 28.2 + 5.436. They were mostly (52.5%) housewives and the Hausa/Fulani ethnic group constituted the majority 141(70.5%). They were mainly, 161(80.5%) Muslims and mostly, 92(46.0%) had tertiary education. About half, 101 (50.5%) belonged to social class 3 and 76 (38.0%) were multiparae. About 84 (42.0%) of them had no female children and 75 (37.5%) had no male children. Majority, 159 (79.5%) were aware that pre-natal gender determination can be assessed using ultrasound. Among them, 130 (65%) were willing to know the babies’ gender. There was statistically significant association between awareness of prenatal ultrasound gender determination and social class of the respondents (P = 0.001). Among them, 55 (43.3%) needed it to prepare for delivery. Conclusion: Awareness and acceptance of ultrasound gender determination is high among the study population. Acceptability depend on the clients’ social class.